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sample purification beads  (New England Biolabs)


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    New England Biolabs sample purification beads
    Expression and <t>purification</t> of recombinant His-MBP-FOXP3(ΔN) protein (A) Schematic flowchart of the protein purification process, including Ni-NTA affinity chromatography followed by HiTrap Heparin HP chromatography. (B) SDS-PAGE analysis of purified fractions during the HiTrap Heparin HP chromatography. The arrow indicates the target protein. M represents the molecular weight marker, with numbers on the right indicating molecular weights in kDa. The gel was stained with Coomassie Blue dye. (C) The HiTrap Heparin HP affinity chromatography profile of the final purified protein. An asterisk indicates the peak corresponding to the desired FOXP3.
    Sample Purification Beads, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 726 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sample purification beads/product/New England Biolabs
    Average 97 stars, based on 726 article reviews
    sample purification beads - by Bioz Stars, 2026-05
    97/100 stars

    Images

    1) Product Images from "Protocol for the genome-wide identification of intrinsic transcription factor binding motifs by mammalian-optimized pull-down sequencing"

    Article Title: Protocol for the genome-wide identification of intrinsic transcription factor binding motifs by mammalian-optimized pull-down sequencing

    Journal: STAR Protocols

    doi: 10.1016/j.xpro.2026.104513

    Expression and purification of recombinant His-MBP-FOXP3(ΔN) protein (A) Schematic flowchart of the protein purification process, including Ni-NTA affinity chromatography followed by HiTrap Heparin HP chromatography. (B) SDS-PAGE analysis of purified fractions during the HiTrap Heparin HP chromatography. The arrow indicates the target protein. M represents the molecular weight marker, with numbers on the right indicating molecular weights in kDa. The gel was stained with Coomassie Blue dye. (C) The HiTrap Heparin HP affinity chromatography profile of the final purified protein. An asterisk indicates the peak corresponding to the desired FOXP3.
    Figure Legend Snippet: Expression and purification of recombinant His-MBP-FOXP3(ΔN) protein (A) Schematic flowchart of the protein purification process, including Ni-NTA affinity chromatography followed by HiTrap Heparin HP chromatography. (B) SDS-PAGE analysis of purified fractions during the HiTrap Heparin HP chromatography. The arrow indicates the target protein. M represents the molecular weight marker, with numbers on the right indicating molecular weights in kDa. The gel was stained with Coomassie Blue dye. (C) The HiTrap Heparin HP affinity chromatography profile of the final purified protein. An asterisk indicates the peak corresponding to the desired FOXP3.

    Techniques Used: Expressing, Purification, Recombinant, Protein Purification, Affinity Chromatography, Chromatography, SDS Page, Molecular Weight, Marker, Staining



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    Expression and <t>purification</t> of recombinant His-MBP-FOXP3(ΔN) protein (A) Schematic flowchart of the protein purification process, including Ni-NTA affinity chromatography followed by HiTrap Heparin HP chromatography. (B) SDS-PAGE analysis of purified fractions during the HiTrap Heparin HP chromatography. The arrow indicates the target protein. M represents the molecular weight marker, with numbers on the right indicating molecular weights in kDa. The gel was stained with Coomassie Blue dye. (C) The HiTrap Heparin HP affinity chromatography profile of the final purified protein. An asterisk indicates the peak corresponding to the desired FOXP3.
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    Image Search Results


    Expression and purification of recombinant His-MBP-FOXP3(ΔN) protein (A) Schematic flowchart of the protein purification process, including Ni-NTA affinity chromatography followed by HiTrap Heparin HP chromatography. (B) SDS-PAGE analysis of purified fractions during the HiTrap Heparin HP chromatography. The arrow indicates the target protein. M represents the molecular weight marker, with numbers on the right indicating molecular weights in kDa. The gel was stained with Coomassie Blue dye. (C) The HiTrap Heparin HP affinity chromatography profile of the final purified protein. An asterisk indicates the peak corresponding to the desired FOXP3.

    Journal: STAR Protocols

    Article Title: Protocol for the genome-wide identification of intrinsic transcription factor binding motifs by mammalian-optimized pull-down sequencing

    doi: 10.1016/j.xpro.2026.104513

    Figure Lengend Snippet: Expression and purification of recombinant His-MBP-FOXP3(ΔN) protein (A) Schematic flowchart of the protein purification process, including Ni-NTA affinity chromatography followed by HiTrap Heparin HP chromatography. (B) SDS-PAGE analysis of purified fractions during the HiTrap Heparin HP chromatography. The arrow indicates the target protein. M represents the molecular weight marker, with numbers on the right indicating molecular weights in kDa. The gel was stained with Coomassie Blue dye. (C) The HiTrap Heparin HP affinity chromatography profile of the final purified protein. An asterisk indicates the peak corresponding to the desired FOXP3.

    Article Snippet: Alternatives: This protocol uses NEBNext Ultra II DNA Library Prep Kit with Sample Purification Beads (NEB #E7103S) to construct library.

    Techniques: Expressing, Purification, Recombinant, Protein Purification, Affinity Chromatography, Chromatography, SDS Page, Molecular Weight, Marker, Staining